High-Concentrated Pullulanase

• Customization: Available
• CAS No.: 9075-68-7
• EINECS: 232-983-9
• Model NO.: PU-750
• Nutritional Value: Enzyme Proteins
• Certification: ISO, FDA
• Packaging Material: 20kg ,30kg Canisters, 1125kg IBC Totes
• Storage Method: Normal
• Shelf Life: >12 Months
• Type: Celluase
• Certificates: Halal, Kosher, ISO Certificates
• Free Sample: Available Upon Request
• Declared Enzyme: Pullulanase
• Appearance: Light to Dark Brown Liquid
• Activity: 7, 000 U/Ml(Minimum)
• Transport Package: 25kg, 30kg Canisters, 1125kg IBC Totes
• Specification: Liquid, activity 7, 000U/ml
• Trademark: Boli
• Origin: Jiangsu, China
• HS Code: 350790
• Production Capacity: 100 Metric Ton/Month

Product Description

PU-750 is a concentrated Pullulanase produced from selected strains of Bacillus licheniformis through submerged fermentation, refining and extraction processes. It is so-named because of its specific function of hydrolysis of pullulan (maltotriose, α-1, 6 glycosidic bond linking polymer). It is an amylase that can specifically cleave the α-1, 6 glycosidic bond of the branching point of amylopectin, and cut out the entire branch structure to form amylose.

PU-750 is primarily used in combination with glucoamylase to produce high glucose syrup and high maltose syrup from liquefied starch.

Application & Benefits 
[High glucose syrup production]
Glucoamylase is very effective in hydrolysis of alpha-1, 4 glycosidic bond; however, glucoamylase is slow in hydrolysis of alpha-1, 6 glycosidic bond, the presence of which constitutes an obstacle in the production of high-glucose syrup. The use of pullulanase can synergize with glucoamylase during the saccharification stage, thus effectively improving the rate of starch hydrolysis, reducing the dosage of glucoamylase, shortening the reaction time and increasing the yield and purity of the glucose.


[For ultra-high-maltose syrup production]
In the production of maltose, alpha-amylase is first used to liquefy the starch, then fungal alpha-amylase or beta-amylase shall be used to decompose starch, so the maltose content of the final product is about 50% to 60%, referred to as a high maltose syrup. The synergistic effect of using pullulanase with alpha-amylase and beta-amylase allows the maltose content in the final product  to reach more than 80%, thereby producing ultra-high-maltose syrup.

[For beer brewing]
In the production of beer, if the starch is not completely decomposed, the fermentable sugar content will be decreased, and the beer made from fermentation will not reach the requirements. The synergistic effect of pullulanase and alpha-amylase enables complete starch decomposition in the raw materials by pullulanase, reducing the content of beta-limit dextrin in the wort and increasing the fermentable sugar content, thus greatly improving fermentation efficacy.

[Other industrial applications]
Adding Pullulanase during the saccharification in the production of MSG, alcohol, vinegar, and soy sauce can improve glucose content and shorten saccharification time.
Using pullulanase in modified starch, such as potato starch, corn starch and sweet potato starch, can increase the content of resistant starch by de-branching, gelatinization and aging process. After de-branching, the starch can produce more short-chain and cross linking through hydrogen bonding and hydrophobic interactions in the regenerative process, aggregation of forming a crystalline structure,i.e. slowly digestible starch. In the natural starch of plants, the amylose only accounts for about 22% to 26%, whilst the majority content is amylopectin. Using pullulanase can cut amylopectin molecules in the alpha-1,6-glycosidic bond, transfer amylopectin into amylase with higher purity.

Effect of pH
PU-750 can work at pH ranging from 4.0 to 5.0, with an optimal pH range of 4.2 to 4.6.

Effect of Temperature
PU-750 can work at temperatures ranging from 50 to 65ºC, with an optimal temperature range of 55 to 62°C.

PACKAGING, STORAGE & SHELF-LIFE

• 25kg, 30kg HDPE plastic drums or 1125kg IBC totes.
• Customized packing is available upon request.
• Typical shelf-life is 18 months if this product is stored at 0- 25°C in its original packaging, sealed and unopened, protected from direct sunlight. Prolonged storage and/or adverse conditions such as higher temperatures may lead to a higher dosage requirement.

SAFE HANDLING OF ENZYMES
Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the Safety Data Sheet.